Escherichia coli remains at the forefront of recombinant protein production, owing to its rapid growth, well-characterised genetics and the ease with which it can be genetically manipulated. The ...

Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

Stay up to date on the latest science with Brush Up Summaries. Finally, although transgenic animals and mammalian cell culture systems promote optimal expression due to correct protein modifications, ...

Non-CHO cell expression systems gain traction as faster, more flexible alternatives for biologics manufacturing.